The sensitivity of qPCR-based assays is one of the primary reasons for their abundant use in scientific laboratories. However, many factors including pipetting technique can influence qPCR assay results. PCR Master mix is routinely used during qPCR set-up but can be challenging to pipette accurately, leading to intra- and inter-assay variability.
Electronic pipettes can be used to quickly and consistently generate accurate Master mixes. Download this application note from Sartorius to explore how electronic pipettes, whether used in conjunction with low retention or standard filter tips, can help ensure reproducible and reliable results when performing PCR-based assays.
