Conventional flow cytometry has relatively limited sensitivity, meaning that heterogeneous cell samples often have to be purified through lysis and wash steps prior to analysis. These steps leave purified populations for analysis, but can cause significant damage to investigated cells, contributing to interexperimental and interlaboratory variation. Technological advancements have enabled no-wash/no-lyse workflows for more precise and faster flow cytometry analysis. No-wash/no-lyse workflows have been used to generate accurate, reproducible results for a variety of applications. 

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